期刊论文

中文

细胞与分子免疫学杂志

1007-8738

2015

31

2

194-198

10.13423/j.cnki.cjcmi.007282

31000091:国家自然科学基金 81072418:国家自然科学基金 :特殊病原体防控湖南省重点实验室项目 :特殊病原体防控湖南省重点实验室项目 2013TT2013:湖南省科研条件创新专项

本校为第一机构

目的观察生殖支原体脂质相关膜蛋白(LAMP)能否诱导胎盘滋养层细胞表达血红素氧合酶1(HO-1), 从而影响细胞因子的产生。方法体外培养胎盘滋养层细胞, 用0.5~5 μg/mL LAMP作用4~12 h。实时定量PCR和Western blot法分别检测HO-1 mRNA和蛋白的表达以及核因子相关因子2(Nrf2)的核转位; 2′, 7′-二氯二氢荧光黄二乙酸酯(H_2 DCFDA)检测活性氧(ROS)产生; 采用N-乙酰半胱氨酸(NAC)或Nrf2 siRNA处理滋养层细胞, 观察其对HO-1表达的影响。采用HO-1的激动剂钴原卟啉(CoPP), 抑制剂锌原卟啉(ZnPP)或HO-1 siRNA处理细胞, ELISA检测处理前后LAMP对诱导肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)分泌的影...

OBJECTIVE: To observe the expression of heme oxygenase-1 (HO-1) in regulation of cytokines response induced by Mycoplasma genitalium-derived lipid-associated membrane proteins (LAMPs) in placental trophoblast cells. METHODS: Placental trophoblast cells were cultured in vitro and stimulated by 0.5-5 mug/mL LAMPs for 4 to 12 hours. Expression of HO-1 mRNA and protein, and nuclear translocation of nuclear factor erythroid-2 related factor 2 (Nrf2) were detected by real-time quantitative PCR and Western blotting, respectively. The intracellular formation of reactive oxygen species (ROS) was detect...