期刊论文

中文

细胞与分子免疫学杂志

1007-8738

2014

30

12

1266-1270

10.13423/j.cnki.cjcmi.007174

国家自然科学基金（31000091,81072418） 特殊病原体防控湖南省重点实验室资助项目（湘科计字[2014]5号,湘教通[2012]312号） 湖南省科研条件创新专项（2013TT2013）

本校为第一机构

目的观察生殖支原体脂质相关膜蛋白（LAMP）能否诱导宫颈上皮细胞表达人β防御素2（hBD-2）,并探讨其可能的调控机制。方法体外培养End1/E6E7人宫颈上皮细胞,用不同浓度的LAMP作用细胞48 h,反转录PCR检测hBD-2 mRNA的表达,Western blot法检测hBD-2蛋白的表达;用Toll样受体2（TLR2）和TLR6中和抗体孵育End1/E6E7细胞,并用TLR2、TLR6和MyD88负显性突变体转染细胞,以明确TLR2、TLR6和MyD88在介导hBD-2表达中的作用;Western blot法检测核因子κB（NF-κB）p65亚基核转位情况,电泳迁移率实验（EMSA）分析LAMP处理前后NF-κB的DNA结合活性;采用NF-κB抑制剂二硫代氨基甲酸吡咯烷（PDTC）处理,观察对hB...

OBJECTIVE: To observe the expression of human beta-defensin-2 (hBD-2) induced by Mycoplasma genitalium-derived lipid-associated membrane proteins (LAMPs) and its potential mechanism. METHODS: Human endocervical epithelial End1/E6E7 cells were cultured in vitro and stimulated by different concentrations of LAMPs for 48 hours, and the expressions of hBD-2 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Toll-like receptor 2 (TLR2) and TLR6 neutralizing antibodies for End1/E6E7 cell cultivation, and dominant negative plasmids for cell transfection were used t...