期刊论文

Mo, Z.C.

中文

细胞与分子免疫学杂志

1007-8738

2011

27

6

685-687

10.13423/j.cnki.cjcmi.006008

国家自然科学基金资助项目（30570958） 湖南省教育厅资助项目（10C1165）

本校为第一且通讯机构

目的:观察过氧化物酶体增殖物激活型受体γ(PPARγ)的激动剂ciglitazone对THP-1巨噬细胞CD36表达及细胞胆固醇流入的影响.方法:以THP-1巨噬细胞为研究对象,用50 mg/L氧化低密度指蛋白(oxLDL)分别与不同浓度的PPAγ激动剂ciglitazone(0、5、10、15 μmol/L)共同孵育THP-1巨噬细胞24 h后,采用液体闪烁计数法检测[3H]胆固醇流入,采用逆转录-聚合酶链式反应和Western blot分别检测CD36的表达.结果:oxLDL及不同浓度的ciglitazone 与THP-1巨噬细胞共孵育后,与对照组比较(20.3%),加ciglitazone的处理组胆固醇流入随着浓度的增加而依次增加,分别为28.6%、37.2%、44.3%、48.7%,细胞CD36的表达上调.结论:c...

AIM: To investigate the effect of ciglitazone on CD36 expression and cholesterol influx in THP-1 macrophage. METHODS: After exposure of the cultured THP-1 macrophage to ciglitazone for 24 h, [(3)H] labeled Cholesterol influx was determined by FJ-2107P typed liquid scintillator. CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively. RESULTS: PPARgamma agonist, ciglitazone, elevated CD36 in both protein and mRNA levels, and increased cholesterol influx in THP-1 macrophage. The levels of cholesterol influx were 20...