期刊论文

中文

微生物学报

0001-6209

2023

63

2

821-833

10.13343/j.cnki.wsxb.20220491

University of South China, USC, (190XQD067) University of South China, USC Education Department of Henan Province, (20B505) Education Department of Henan Province National College Students Innovation and Entrepreneurship Training Program, (X202210555245) National College Students Innovation and Entrepreneurship Training Program

本校为第一且通讯机构

【目的】本研究筛选出弱酸性环境下利用无机碳源进行高效脱氮的氢自养微生物,探究不同无机碳源对体系反硝化能力的影响,以及长期驯化过程中反应器内水质参数、微生物群落结构和脱氮周期变化规律。【方法】氢自养微生物的驯化采用一种成本低廉、气密性优良、可计算氢气利用率的序批式反应器,通过及时向装置内补充氢气、无机碳源、营养液和硝酸盐对微生物进行连续驯化。【结果】驯化的微生物利用NaHCO_3和CO_2作为混合无机碳源对硝酸盐的脱氮效果要优于单一使用NaHCO_3;在环境温度为20 ℃,pH为6.3-7.0,硝态氮初始投加量为15 mg-N/L时, NO_3~——N最高反应速率为1.374 mg-N/(L·h),氢气最高利用率为...

[Objective] We screened out hydrogenotrophic microorganisms using inorganic carbon sources for high efficiency in denitrification under weak acidic conditions, to investigate the effects of different inorganic carbon sources on the denitrification capacity of the system, and to explore the variation in water quality parameters, microbial community structure, and denitrification cycle in the reactor during long-term cultivation. [Methods] We developed a sequential batch reactor for continuous cultivation of microorganisms by supplying hydrogen, ...