In order to explore the characteristics on growth and carbonic anhydrase(CA)producing strain of Bacillus safensis,the effects of factors including initial pH,inoculation size,temperature,shaker rotational speed,and metal ions of Zn2+,Co2+,Cd2+,Mn2+,Mg2+,and Ca2+on the bacterial growth and CA activity were investigated by single factor experiment.The results showed that the optimal conditions for the growth and CA production of this strain were different.The bacteria grew fastest at 30℃,and the enzyme activity was the highest at 45℃.Furthermor...