期刊论文

Amelioration of DSS-induced ulcerative colitis in mice by Chlamydia muridarum

中文

微生物学报

0001-6209

2020

60

4

695-702

10.13343/j.cnki.wsxb.20190279

2018JJ2326:湖南省自然科学基金 2018XJXZ379:南华大学大学生研究性学习和创新性实验计划 2018XJXZ382:南华大学大学生研究性学习和创新性实验计划

本校为第一机构

【目的】探讨鼠衣原体(Chlamydia muridarum)对小鼠溃疡性结肠炎的作用。【方法】取15只雌性C57BL/6J小鼠随机分为3组,每组5只动物,分别为空白对照组(Control)、肠炎模型组(DSS)、实验组(CM+DSS)。选取CM+DSS组小鼠予以2×10~5 IFU的鼠衣原体灌胃处理,并在其感染后第29天开始,给予DSS组和CM+DSS组的小鼠2% DSS饮水,持续5 d,每天监测小鼠体重和肠炎疾病评分,实验结束后检测小鼠结肠长度和结肠组织炎性改变。【结果】肠炎模型组的小鼠均表现出典型的肠炎症状(包括体重减轻、肠炎疾病评分、结肠长度和组织炎性改变);而经鼠衣原体预处理的小鼠(CM+DSS组)肠炎症状显著减轻,表现在肠炎疾病评分降低,体...

[Objective] To study the effect of Chlamydia muridarum on the ulcerative colitis induced by dextran sulfate sodium (DSS) in mice. [Methods] In total 15 female C57BL/6J mice were randomly divided into 3 groups: control group, DSS group, experimental group (CM+DSS group). The mice of CM+DSS group were administrated with 2×10~5 IFU Chlamydia muridarum by gavage. After 28 days, the mice of the DSS group and CM+DSS group drank 2% DSS water for 5 days, then they were monitored for body weight and disease activity index, the colon length of all mice,...