期刊论文

中文

微生物学报

0001-6209

2016

56

2

317-326

10.13343/j.cnki.wsxb.20150380

acknowledge the support from the National Natural Science Foundation of China;the Program for Changjiang Scholars and Innovative Research Team in University;State Key Laboratory of Polymer Materials Engineering

本校为第一机构

【目的】分离鉴定噬菌体,对其生物学特性进行研究,并筛选候选毒株为防控牛源无乳链球菌的感染提供依据。【方法】分别采用从牛奶或环境中分离、溶原菌诱导两种方法分离鉴定无乳链球菌噬菌体,利用双层琼脂平板法纯化。将新分离鉴定毒株与前期已分离鉴定的源自乳腺炎牛奶的无乳链球菌噬菌体JX01进行分析和比较,包括噬菌体透射电镜形态观察、对55株无乳链球菌和其他细菌的宿主谱鉴定、噬菌体基因EcoR I、Sal I、Xba I或Pst I的酶切图谱、最适MOI、吸附曲线和一步生长曲线、不同保存条件下的稳定性等。【结果】分离鉴定的3株噬菌体LYGO9、HZ04和pA11 (诱导自牛源菌株HAJL2011070601)与JX01比对分析,...

[Objective] To isolate and identify Streptococcus agalactiae phages and screen candidate phages to control infection caused by bovine S. agalactiae. [Methods] We used two methods for isolation of S. agalactiae phages, namely （1） isolation of phages from milk and environmental samples, and （2） isolation of phages via induction of lysogens with Mitomycin C. Double-layer agar culture method was used to purify phages. Then the newly obtained phages, with S. agalactiae phage JX01 isolated from mastitis milk, were comparatively analyzed in the fo...