期刊论文

Microwave Mutation Breeding for Urease-Producing Sporoscarcina pasteurii

中文

微生物学杂志

1005-7021

2016

36

3

20-23

10.3969/j.issn.1005-7021.2016.03.004

湖南省教育厅科学研究重点项目（13A083）；

本校为第一机构

巴氏芽胞杆菌是目前微生物诱导碳酸钙沉淀(MICP)方法中应用最为热门的一种细菌。为提高巴氏芽胞杆菌尿素分解以及矿化能力,以巴氏芽胞杆菌YB-B为出发菌株,采用微波诱变育种技术,通过诱变菌株特性筛选及其遗传稳定性检测,成功选育出2株突变菌株YB-3和YB-4。与出发菌株相比,诱变菌株尿素分解能力较原菌株提高1.5倍左右,矿化能力提高114%。诱变菌株具有生长速度快,环境适应性强,矿化能力高等优点,这为MICP更广层次的应用奠定了坚实的基础。

Sporoscarcina pasteurii is known as the most popular bacterium applied to microbial induced calcium car-bonate precipitation （MICP） method in the present researches. In order to improve its ability of urea decompositionand biomineralization, two mutant strains YB-3 and YB-4 has been successfully bred based on the S. pasteurii strainYB-B adopting microwave breeding technique, and determined and tested their genetic stability through special screen-ing of the mutant strains. The capacity of urea decomposition had been increased 1.5 times or so ...