期刊论文

中文

微生物学报

0001-6209

2015

55

4

492-500

10.13343/j.cnki.wsxb.20140435

国家自然科学基金项目（81001318,30971394） 湖南省教育厅科学研究青年项目（13B099,12B109） 湖南省自然科学基金（13JJ4072） 南华大学博士启动基金（2014XQD42） 特殊病原体防控湖南省重点实验室【湘科计字（2014）5号＋湘教通（2012）312号】~~

本校为第一机构

【目的】初步探讨与单纯疱疹病毒糖蛋白D 竞争结合疱疹病毒侵入介体的淋巴毒素类似物(lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells,LIGHT)在抗衣原体感染免疫及介导衣原体生殖道病理损伤过程的作用。【方法】用1 × 10~4 IFUs 的MoPn 经生殖道感染野生型(wild type,wt) 、LIGHT KO 小鼠,每组一半小鼠于感染后49d,再次感染相同剂量的MoPn。每隔3 - 4 d取生殖道分泌物,测定其中衣原体包涵体的数量。初次感染后80d,处死小鼠,眼眶取血,分离血清,用间接免疫荧光法测定其中抗体类型及效价; 同时分离生殖道,肉眼观察其输卵管、子宫角水肿...

OBJECTIVE: To study the role of lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells (LIGHT) in the development of protective immunity and pathology during Chlamydia Muridarum urogenital infection in mice. METHODS: C57BL/6J wild type (wt) and mice deficient in LIGHT (LIGHT KO) were inoculated intravaginally with 1 x 10(4) IFUs of live C. muridarum organisms. Half mice of each group were reinfected on day 49 after primary infection. We took mice vaginal swabs every 3 or 4 days to monitor live organism shedding. On day 80 after the primary infecti...