期刊论文

Tang, G.

中文

微生物学报

0001-6209

2010

50

12

1657-1663

10.13343/j.cnki.wsxb.2010.12.012

(2008ZX10004-001):国家科技重大专项项目

本校为第一且通讯机构

【目的】优化鹦鹉热嗜衣原体（Chlamydophila psittaci,C.psittaci,Cps）禽鸟株分离培养技术,建立Cps呼吸道感染的小鼠模型。【方法】从禽鸟肝组织中抽提总DNA,PCR法体外扩增Cps ompA基因,初步鉴定Cps阳性标本;同时将阳性标本的肝组织匀浆液接种到HeLa和Vero细胞中培养,Giemsa和免疫荧光染色法鉴定衣原体包涵体。将临床株扩大培养后,用2×104、2×105、2×106 IFU三个剂量鼻内接种小鼠,分别于感染后5 d和10 d处死小鼠,显微镜观察受染小鼠各脏器病理变化。【结果】采用PCR扩增Cps ompA基因,从100只禽鸟标本中检测到阳性Cps标本6例（6%）,并成功地在HeLa及Vero细胞中培养出3例,且Vero细胞内的衣原...

OBJECTIVE: To optimize the isolation and culture technique of Chlamydophila psittaci avian strains and to establish an animal model infected with C. psittaci. METHODS: C. psittaci ompA gene was amplified from DNA extracted from bird livers by polymerase chain reactions (PCR). For the PCR positive avian samples, the liver tissues were homogenized and used to incubated with HeLa or Vero cell monolayers for 72 h in different dilutions, and chlamydia inclusion bodies were detected by immunofluorescence or Giemsa staining. Different dose of the avian strains (2 x 10(4), 2 x 10(5), 2 x 10(6) IFUs) w...