期刊论文

Chen, L.L.

中文

微生物学报

0001-6209

2006

46

2

214-218

10.13343/j.cnki.wsxb.2006.02.010

湖南省科技厅重点资助项目（04SK2003） 致谢 感谢南华大学遗传学教研室郑济芳博士在数据分析方面提供的帮助.

本校为第一机构

从中国不同城市收集疑为沙眼衣原体（Ct）感染的泌尿生殖道标本323份，巢式PCR扩增Ct omp1l基因片段（包括4个变异区），测定其中96份阳性标本omp1基因序列，根据同源性分型并分析其多态位点；根据氨基酸序列，用Mega3软件构建进化树，分析临床株与相应参考株之间的亲缘关系。从96份沙眼衣原体阳性标本中，检出28种基因变体，其中E型最常见；同时发现ctE、F型omp1基因高度保守，而其它基因型都显示一定的变异性。进化树分析发现，各临床株与相应参考株之间遗传距离较近。实验结果表明沙眼衣原体omp1基因呈现较大的多态性，可为其疫苗的研制及感染的防治提供重要的实验依据。

A method for detection and genotyping of genital Chlamydia trachomatis infections based on omp1 gene amplification and sequencing was developed, and the character of omp1 gene of Chlamydia trachomatis was analysed. Urethral or endocervical specimens were collected from 323 patients attending STD clinics in Hengyang, Shanghai, Guangzhou and Jiangmen from November, 2003 to May, 2004. DNA was extracted by usual method, and an approximately 980bp fragment from the major outer membrane protein (omp1) gene of Chlamydia trachomatis was amplified by nested polymerase chain reaction (nPCR). The PCR pro...