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Target-based metabolomics for the quantitative measurement of 37 pathway metabolites in rat brain and serum using hydrophilic interaction ultra-high-performance liquid chromatography-tandem mass spectrometry

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成果类型:
期刊论文
作者:
Chen, Jiahui;Hou, Waner;Han, Bo;Liu, Guanghui;Gong, Jin;Li, Yemeng;Zhong, Danmin;Liao, Qiongfeng;Xie, Zhiyong
通讯作者:
Liao, Q.
作者机构:
Guangzhou University of Chinese Medicine<&wdkj&>Shihezi University<&wdkj&>Sun Yat-sen University
[Chen, Jiahui; Hou, Waner; Liu, Guanghui; Gong, Jin; Liao, Qiongfeng] Guangzhou University of Chinese Medicine
[Han, Bo] Shihezi University
[Li, Yemeng; Zhong, Danmin; Xie, Zhiyong] Sun Yat-sen University
通讯机构:
[Liao, Qiongfeng] Guangzhou Univ Chinese Med, Sch Chinese Mat Med, Guangzhou 510407, Guangdong, Peoples R China.
[Xie, Zhiyong] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China.
[Xie, Zhiyong] Univ South China, Cooperat Innovat Ctr Mol Target New Drug Study, Hengyang 421001, Hunan, Peoples R China.
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, China
语种:
英文
关键词:
Target-based metabolomics;Quantitative;HILIC–MS;Amino acids;neurotransmitters;purines and pyrimidines
期刊:
Analytical and Bioanalytical Chemistry
ISSN:
1618-2642
年:
2016
卷:
408
期:
10
页码:
2527-2542
基金类别:
National Natural Science Foundation of China [81473319, 81473540]; Science and Technology Project of Guangdong Province [2014A02020902, 2014A020221027, 2015A030401031]; Natural Science Foundation of Guangdong Province [2015A030313123]
机构署名:
本校为通讯机构
院系归属:
药学与生物科学学院
摘要:
Amino acids, neurotransmitters, purines, and pyrimidines are bioactive molecules that play fundamental roles in maintaining various physiological functions. Their metabolism is closely related to the health, growth, development, reproduction, and homeostasis of organisms. Most recently, comprehensive measurements of these metabolites have shown their potential as innovative approaches in disease surveillance or drug intervention. However, simultaneous measurement of these metabolites presents great difficulties. Here, we report a novel quantitative method that uses hydrophilic interaction ultra-high-performance liquid chromatography-tandem mass spectrometry (HILIC-UPLC-MS/MS), which is highly selective, high throughput, and exhibits better chromatographic behavior than existing methods. The developed method enabled the rapid quantification of 37 metabolites, spanning amino acids, neurotransmitters, purines, and pyrimidines pathways, within 6.5 min. The compounds were separated on an ACQUITY UPLC&reg;BEH Amide column. Serum and brain homogenate were extracted by protein precipitation. The intra- and interday precision of all of the analytes was less than 11.34 %, and the accuracy was between -11.74 and 11.51 % for all quality control (QC) levels. The extraction recoveries of serum ranged from 84.58 % to 116.43 % and those of brain samples from 80.80 % to 119.39 %, while the RSD was 14.61 % or less for all recoveries. This method was used to successfully characterize alterations in the rat brain and, in particular, their dynamics in serum. The following study was performed to simultaneously test global changes of these metabolites in a serotonin antagonist p-chlorophenylalanine (PCPA)-induced anxiety and insomnia rat model to understand the effect and mechanism of PCPA. Taken together, these results show that the method is able to simultaneously monitor a large panel of metabolites and that this protocol may represent a metabolomic method to diagnose toxicological and pathophysiological states. &copy;Springer-Verlag Berlin Heidelberg 2016.

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