The herbicide glyphosate, N-(phosphonomethyl)glycine, has been widely used in the past 40 years, and has had many adverse effects on human health. Here, we constructed a convenient "on-off-on" fluorescent platform for detection of glyphosate via Cu(2+) modulated squaraine dye fluorescence quenching. The squaraine dye F-0 exhibited strong fluorescence, which could be quenched by the addition of Cu(2+). However, the addition of glyphosate restored the fluorescence intensity of F-0 due to the formation of a Cu(2+)-glyphosate complex. F-0 was utilized as a fluorescent probe for the quantitative de...