期刊论文

Effect of high sucrose/high fat diet and Streptozotocin on amylon composition and musclin expression of skeletal muscles in Chinese Banna mini pigs

中文

中国糖尿病杂志

1006-6187

2017

25

12

1135-1140

10.3969/j.issn.1006-6187.2017.12.016

湖南省教育厅科研项目（15C1202、12C0341） 湖南省科技厅重点研发项目（2016DK2011）

本校为第一机构

目的 观察高糖高脂联合低剂量STZ对版纳微型猪骨骼肌肌肉素(M usclin)表达的影响.方法 高糖高脂联合低剂量STZ诱导版纳微型猪T2DM 模型,每月末测定血糖、血脂、胰岛素及Musclin水平变化.12个月末处死动物,HE和PAS及骨骼肌糖原含量(M2酶标仪,比色法)测定,ELISA检测血清Musclin含量,Western blot检测肌肉Musclin蛋白表达. 结果 喂养12月后,与对照(Con)组比较,模型(HSFD)组血糖[(4.75 ± 0.2)vs(19.28 ± 2.24)mmol/L]、TG[(0.82 ± 0.14)vs(2.46 ± 0.29) mmol/L]和TC[(2.29 ± 0.35)vs(8.93 ± 0.43)mmol/L]升高(P＜0.05),胰岛素缺乏[(8.25 ± 1.04)vs (3.81 ± 0.39)mU/L,P＜0.05];骨骼肌萎缩,糖...

Objective To investigate the effect of high-fat/high-sucrose/streptozotocin(STZ)on musclin levels in the skeletal muscles of Chinese Banna mini pigs. Methods T2DM model of Chinese Banna mini pigs were induced by feeding with high-fat/high-sucrose and treating with Streptozotocin (HSFD group). Plasma glucose,lipids,insulin,and musclin were measured at the end of each month. After following up for 12 months,all the Banna mini pigs were killed. Skeletal muscles were observed by HE staining,periodic acid Schiff reaction(PAS)staining. The glycogen c...