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A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu2+ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly

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成果类型:
期刊论文
作者:
Liu, Zhen;Liu, Chen;He, Liqiong;Liu, Jinquan*;Li, Le;...
通讯作者:
Liu, Jinquan;Xiao, XL
作者机构:
[Yang, Shengyuan; Tan, Yan; Liu, Xing; Liu, Jinquan; He, Liqiong; Li, Le; Liu, JQ; Xiao, Xilin; Xiao, XL; Liu, Zhen] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
[Liu, Chen] Univ South China, Sch Chem & Chem Engn, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haza, Hengyang 421001, Peoples R China.
[Xiao, Xilin; Xiao, XL] Hunan Univ, State Key Lab Chemo & Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构:
[Liu, JQ; Xiao, XL ] U
Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
Hunan Univ, State Key Lab Chemo & Biosensing & Chemometr, Changsha 410082, Peoples R China.
语种:
英文
关键词:
Cu2+ detection;lambda-exonuclease-assisted target recycling;mismatched catalytic hairpin assembly;cascade signal amplification;environmental pollutant
期刊:
Biosensors
ISSN:
2079-6374
年:
2023
卷:
13
期:
10
页码:
918-
基金类别:
Conceptualization, methodology, investigation, experimentation, formal analysis, and writing—original draft, Z.L.; investigation, software, and formal analysis, C.L.; investigation, software, and formal analysis, L.H.; supervision, funding acquisition, and writing—review and editing, J.L.; investigation and formal analysis, L.L.; formal analysis and visualization, S.Y.; validation and funding acquisition, Y.T.; investigation and formal analysis, X.L.; supervision, validation, methodology, funding acquisition, and writing—review and editing, X.X. All authors have read and agreed to the published version of the manuscript. This research was funded by the Research Foundation of the Education Bureau of Hunan Province, China (19A441); the Natural Science Foundation of Hunan Province (2021JJ30576 and 2023JJ50139); the Open Project Program of the State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University (2021017); and the Science and Technology Planning Project of Hengyang City, Hunan Province, China (202010031546).
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
公共卫生学院
摘要:
Herein, an ultrasensitive DNAzyme-based fluorescence biosensor for detecting Cu2+ was designed using the cascade signal amplification strategy, coupling lambda-exonuclease-assisted target recycling and mismatched catalytic hairpin assembly (MCHA). In the designed detection system, the target, Cu2+, can activate the Cu2+-dependent DNAzyme to cause a cleavage reaction, releasing ssDNA (tDNA). Then, tDNA binds to hairpin DNA (H0) with an overhanging 5 '-phosphorylated terminus to form dsDNA with a blunt 5 '-phosphorylated terminus, which activates the dsDNA to be digested by lambda-Exo and releas...

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