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Novel and label-free colorimetric detection of radon using AuNPs and lead(II)-induced GR5 DNAzyme-based amplification strategy

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成果类型:
期刊论文
作者:
Liu, Hongwen;Chen, Yating;Song, Chunli;Tian, Gang;Li, Shiya;...
通讯作者:
Lv, Changyin
作者机构:
[Chen, Yating; Li, Shiya; Yang, Guiying; Lv, Changyin; Liu, Hongwen; Song, Chunli; Tian, Gang] Univ South China, Coll Publ Hlth, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Lv, Changyin] U
Univ South China, Coll Publ Hlth, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
DNAzyme;Gold nanoparticles;Lead;Radon;Spectrophotometry
期刊:
Analytical and Bioanalytical Chemistry
ISSN:
1618-2642
年:
2018
卷:
410
期:
17
页码:
4227-4234
基金类别:
Funding information The authors received support from the National Natural Science Foundation of China (No. 81473021) and Graduate Student Research Innovation Project in Hunan Province (No. CX2017B557).
机构署名:
本校为第一且通讯机构
院系归属:
公共卫生学院
摘要:
Radioactive radon decays into a stable daughter product, 210Pb, which was used as the detection target to determine the radon radiation dose in a new technique. Pb2+ triggers DNAzyme to cleave a molecular beacon (MB), resulting in the stem–loop structure opening and forming two single DNA strands (ssDNA). The ssDNA binds to unmodified gold nanoparticles and effectively prevents their aggregation in a salt solution. The detached enzyme strands continue to complement the remaining MB to amplify the response signal. The method proposed in this st...

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