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IGF-1 inhibits palmitic acid-induced mitochondrial apoptosis in macrophages

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成果类型:
期刊论文
作者:
Tang, Wanying;Zhang, Ming;Wang, Yu;Ma, Dan;Hu, Mi;...
通讯作者:
Ouyang, XP;Zhao, GJ
作者机构:
[Tang, Wanying; Ouyang, Xinping; Lin, Huiling; Ouyang, Yuxin; Zhang, Yangkai; Hu, Mi] Univ South China, Inst Neurosci Res, Dept Physiol, Hengyang Key Lab Neurodegenerat & Cognit Impairmen, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
[Tang, Wanying; Ouyang, Xinping; He, Pingping] Hunan Normal Univ, Med Coll, Res Ctr Reprod & Translat Med Hunan Prov, Dept Physiol, Changsha 410081, Hunan, Peoples R China.
[Wang, Yu; Zhao, GJ; Zhao, Guojun; Zhang, Ming] Guangzhou Med Univ, Affiliated Hosp 6, Inst Cardiovasc Dis, Area B24,Yinquan Rd, Qingyuan 511500, Guangdong, Peoples R China.
[Ma, Dan] Zunyi Med Univ, Sch Pharm, Zunyi 563000, Guizhou, Peoples R China.
[Jiang, Weiwei] Southern Med Univ, Zhujiang Hosp, Dept Organ Transplantat, Guangzhou 510280, Guangdong, Peoples R China.
通讯机构:
[Zhao, GJ ] G
[Ouyang, XP ] U
Univ South China, Inst Neurosci Res, Dept Physiol, Hengyang Key Lab Neurodegenerat & Cognit Impairmen, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
Guangzhou Med Univ, Affiliated Hosp 6, Inst Cardiovasc Dis, Area B24,Yinquan Rd, Qingyuan 511500, Guangdong, Peoples R China.
语种:
英文
关键词:
caspase 3;cytoplasm protein;hoe 33342;palmitic acid;parkin;phosphatase;reactive oxygen metabolite;somatomedin;tensin;insulin;palmitic acid;reactive oxygen metabolite;somatomedin C;ubiquitin protein ligase;apoptosis;Article;cell isolation;cell viability assay;enzyme activity;flow cytometry;human;human cell;immunofluorescence;macrophage;mitochondrial membrane potential;mitochondrion;mitophagy;survival rate;THP-1 cell line;Western blotting;apoptosis;metabolism;Apoptosis;Insulin;Insulin-Like Growth Factor I;Mitophagy;Palmitic Acid;Reactive Oxygen Species;Ubiquitin-Protein Ligases
期刊:
MOLECULAR MEDICINE REPORTS
ISSN:
1791-2997
年:
2023
卷:
28
期:
6
基金类别:
The present study was supported by natural Science Foundation of china (grant no. 82170485), natural Science Foundation of Hunan Province, china (grant nos. 2019JJ40 249 and 2023JJ30426) and university‑industry cooperation education Project of education department (grant no. 202002138007).
机构署名:
本校为第一且通讯机构
院系归属:
医学院
摘要:
insulin growth factor‑1 (iGF‑1) is an endocrine regulator that plays an important role in normal growth and development. iGF‑1 mediated effects may result in protecting macrophages from immunometabolic response. However, it is unclear whether iGF‑1 has a protective effect on fatty acid‑induced macrophages damage. in the present study, THP‑1 cells were differentiated into macrophages and stimu‑ lated with palmitic acid (Pa) in the absence or presence of iGF‑1. Macrophages apoptosis was measured by cell counting Kit‑8 assay, flow cytomet...

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