This paper firstly presents the operation principle of individually ventilated cages (IVC) system. Measurements on micro-environment of IVC system in laboratory animal room at University of South China are conducted to attain the internal environment parameters (Temperature, humidity, air velocity, air cleanliness and noise). Research results show that internal micro-environment parameters basically meet national standards about the barrier environment, but internal air distribution is uneven. In IVC system, air velocities in the edge side are higher than in the middle side, and the maximum ai...