期刊论文

Detection of Urinary Mycoplasma by Using A7 Agar Medium Method

中文

皮肤性病诊疗学杂志

1674-8468

2003

10

3

170-171

10.3969/j.issn.1674-8468.2003.03.011

本校为第一机构

目的:评价A7琼脂法在检测泌尿生殖道标本中解脲脲原体(Ureaplasma urealyticum,Uu)和人型支原体(Mycoplsma hominis,Mh)中的意义,为临床诊断Uu和Mh的感染提供实验依据.方法:取接种了泌尿生殖道标本的运送培养基直接接种于A7琼脂,在5%CO2和95%N2的气体环境中培养24h～48 h后,低倍镜下观察特征性菌落,计数其菌落形成单位(Colony Forming Units,CFU).结果:286例泌尿生殖道标本中分离到Uu102例,Mh29例,混合感染27例,Uu计数≥104CFU者75例,Mh计数≥104CFU者24例.结论:A7琼脂法简便可行,结果直观可信,适用于临床泌尿生殖道中Um、Mh的检测.

Objective:To evaluate the A7 agar medimn method to be applied to detect ureaplasma urealyticum(Uu) and mycoplasma hominis(Mh) in urinary tract and provide laboratory test for clinical diagnosis. Methods:Shipping medium with clinical specimens in it was inoculated in A7 agar plate. Incubated under 5% CO 2/95% N 2 for 24 to 48 hours,specific colonies were observed by microscope and colony forming units(CFU) were counted.Results: Of all 286 samples studied, 102 U. urealytlcum,29 M. hominis and 27 co-infection have been isolated in A7 agar. U. urealyticum CFU of 75 cases were more than or equal to...