The authors studied the effect of Taijiquan exercise on serum supplements 3 （C3） and 4 （CA） and overall supplement activity in grade 4 female college students, so as to provide immunological experiment criteria for developing Taijiquan aerobic exercise among senior college students. The authors randomly selected 40 class 2001 female students studying at Nursing College of Nanhua University, and divided them into 4 groups, namely, control group and experiment groups 1, 2 and 3. The experiment groups were trained for Taijiquan for 1, 2 and 3 times respectively every week. After 0, 2, 4, 6, 8,10 and 12 weeks, hlood was sampled to separate serum, and the quality concentration of supplements C3 and CA as well as overall supplement activity in serum before and after the training were measured respectively by applying the immunonephelometer approach and the 50% hemolysis approach （CH50）. The results revealed that the quality concentration of sexton supplements C3 and CA as well as overall supplement activity in those trainees who did Taijiquan exercise 3 times a week were higher than those in people in the control group and those trainees who did Taijiquan exercise once or twice a week, and were significantly different（ P 〈 0.05） from those in people in the control group and those trainees who did Taijiquan exercise once a week in the 10th and 12th weeks, indicating that doing Taijiquan aerobic exercise consistently and moderately can enhance overall supplement activity and the ability of the bodies of female senior college students to compose C3 and CA, thus enhancing non- specific anti- infection immunity and enhancing immunity response level.