期刊论文

ERK1/2 pathway is involved in up-regulation of mesenteric artery α1 receptor by minimally modified low density lipoprotein

中文

中华生物医学工程杂志

1674-1927

2018

24

1

1-6

10.3760/cma.j.issn.1674-1927.2018.01.001

国家自然科学基金资助项目(81202535)%湖南省科技厅自然科学基金资助项目(2018JJ6097)%湖南省卫生计生委资助课题(B2017183)%郴州市科技局资助项目(jsyf2017025)China National Natural Science Foundation(81202535)%Natural Science Fund Project of Hunan Science and Technology Department(2018JJ6097)%Hunan Provincial Health and Family Planning Commission Fund Project(B2017183)%Chenzhou Science and Technology Bureau Fund Project(jsyf2017025)

本校为第一机构

目的 探讨ERK1/2通路在弱氧化型低密度脂蛋白(mmLDL)上调小鼠肠系膜动脉α1受体中的作用.方法 72只小鼠随机分成3组,正常对照组(小鼠尾静脉注射生理盐水),mmLDL组(小鼠尾静脉注射mmLDL),U0126干预组(小鼠尾静脉注射mmLDL及腹腔注射ERK1/2通路特异性抑制剂U0126),各24只.微血管肌张力描记仪观察去甲肾上腺素(NA)引起的小鼠肠系膜动脉收缩量效曲线变化,RT-PCR、Western Blotting分别检测α1受体、TNF-α、IL-1β和p ERK1/2的表达,Elisa试剂盒检测血清中TNF-α和IL-1β浓度水平.结果 与正常对照组比较,mmLDL组Emax、pEC50值显著升高,α1受体介导血管收缩增强,α1受体mRNA和蛋白表达、pERK1/2蛋白磷...

Objective To investigate the effect of ERK1/2 pathway in the up-regulation of mesenteric artery α1 receptor by minimally modified low density lipoprotein (mmLDL) in mice. Methods Seventy-two mice were randomly divided into 3 groups:the normal control group(injection of normal saline via caudal vein in mice),mmLDL group(injection of mmLDL via caudal vein in mice),U0126 intervention group(injection of mmLDL via caudal vein and intraperitoneal injection of ERK1/2 pathway specific inhibitor U0126 in mice)(n=24 each). Microvascular myograph was emp...