期刊论文

中文

中华生物医学工程杂志

1674-1927

2000

6

2

1-3

10.3760/cma.j.issn.1674-1927.2000.02.002

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目的　研究人脐静脉内皮细胞 (HUVEC)在支架上的粘附生长的影响因素 ,寻求最佳细胞粘附条件 .方法　HUVEC培养按文献方法进行 .利用免疫组化方法 (Sp法 )检测Ⅷ因子以鉴定细胞纯度 ,分别比较细胞代数、Fibronectin、poly-L -lysine及Ca2 + 浓度对HUVEC在支架上的粘附率的影响 .结果　 (1)第 2、3、4代HUVEC在裸支架上的粘附率分别为 (2 0 .0 5± 2 .1) %、(16 .12± 4.0 2 ) %、(10 .6 1± 5 .0 1) % (n =4) ;(2 )Fibronectin(n =6 )、poly-L -lysine(n =4)处理支架 ,HUVEC的粘附率分别为 (6 4.3± 6 .19) %、(39.88± 1.31) % ;(3)与 1mM、2mM、3mM、4mM、5mMCa2 + 相对应的细胞粘附率分别为 (...

Objective To study the influential factors on the adhesive rateio of human umbilical vein endothelial cells (HUVECs) on Ni-Ti intravascular stents.To seek for the optimum cell adhesive conditions.Methods HUVECs were cultured as Jaffe did.HUVECs were identified by Ⅷ factor detecting with immunohistochemical method(SP).The influence of different generation、Fibronection、poly-L-lysine and the different concentration of Ca 2+ on the adhesive ratio of HUVEC on the stent was studied.Results (1)Adhesive ratios of the 2nd、3rd、4th generation were (2...