期刊论文

中文

微生物学通报

0253-2654

2015

42

8

1561-1568

10.13344/j.microbiol.china.150013

国家自然科学基金项目（No.81100648） 湖南省自然科学基金项目（No.13JJ4072） 湖南省教育厅优秀青年基金项目（No.12B109,13B099） 分子靶标新药研究协同创新中心项目[No.湘教通（2014）405号]

本校为第一机构

【目的】探讨鼠衣原体（Chlamydia muridarum,Cm）标准株与减毒株全基因组序列中存在的差异,筛选毒力基因并建立不同基因型的单克隆菌株,为后续致病机制的研究奠定基础。【方法】将Cm标准株G0和减毒株G28以高通量测序法进行全基因组测序,通过全基因组序列比对分析并筛选潜在的毒力相关基因;经空斑形成实验（Plaque assay）在混合菌G0和G28中大量挑取空斑,以毒力靶基因的PCR测序鉴定从G0和G28中筛选含有不同毒力基因型的单克隆菌株。【结果】全基因测序结果显示Cm G0与G28的TC0412、TC0237和TC0668基因明显不同。通过挑取空斑初步筛选了111个空斑样品,通过3个毒力基因的PCR测序鉴定最终获得了G0和...

[Objective] To find difference in whole genome sequence between Chlamydia muridarum standard strain and attenuated strain, screening virulence gene and establish the corresponding isogenic organisms with different genotype, and it may lay a foundation for analyzing pathogenic mechanism. [Methods] C. muridarum strain Nigg G0 and attenuated strain G28 were sequenced by deep sequcencing. Virulence-associated genes were identified by whole genome sequencing and alignment. A plaque-forming assay was used to isolate many plaques from both CM.G0 and C...