To tract the intracellular localization of the hypothetical protein CT058 in the Chlamydia trachomatis-infected cells. The gene ct058 was expressed as GST-CT058 fusion protein in E. coli and its antibody was prepared by immunizing mouse with the fusion protein. Then, the anti-GST-CT058 antibody was used to localize the endogenous protein in cells infected by C. trachomatis serovar L2 using indirect immunofluorescence assay （IFA）. Western blot was carried out to detect whether CT058 presents in the purified elementary body （EB） or reticulate...
