期刊论文

中文

食品安全质量检测学报

2095-0381

2020

11

12

4091-4096

10.19812/j.cnki.jfsq11-5956/ts.2020.12.061

湖南省自然科学基金（2015SK20012）~~；

本校为第一机构

目的 建立超高效液相色谱法(ultra-performance liquid chromatography,UPLC)同时测定蜂蜜中双甲脒及其代谢物(2,4-二甲基苯胺)残留量的方法.方法 样品经0.1 mol/L NaOH溶液提取、正己烷-异丙醇(2:1,V:V)混合液萃取,再采用ACQUITY UPLC?BEH C18(2.1 mm×100 mm,1.7μm)色谱柱进行分离,以乙腈和20 mmol/L乙酸铵溶液作为流动相,柱温30℃、流速为0.20 mL/min,检测器波长在235 nm处进行检测,外标法定量.结果 双甲脒及2,4-二甲基苯胺标准曲线在2.0～500.0 ng/mL范围内呈良好的线性关系,线性相关系数均大于0.999,方法检出限双甲脒为0.27μg/kg,2,4-二甲基苯胺为0.42μg/kg,以样品加标做回收率测定,双甲...

Objective To establish a method for the determination of amitraz and its metabolite(2,4-dimethylaniline)residues in honey by ultra-performance liquid chromatography.Methods The samples were diluted with 0.1 mol/L sodium hydroxide,extracted with n-hexane-isopropanol(2:1,V:V),and then separated by ACQUITY UPLC®BEH C18(2.1 mm×100 mm,1.7μm)with ammonium acetate(20 mmol/L)/Acetonitrile as the gradient eluent with a flow rate of 0.20 mL/min at 30℃.A diode array detector was used to detect at 235 nm.The external standard method was used for quanti...