期刊论文

中文

食品安全质量检测学报

2095-0381

2019

10

1

195-203

10.3969/j.issn.2095-0381.2019.01.033

国际原子能机构合作研究基金（CRPD5203918859）~~；

本校为第一机构

目的 建立贝类产品中呋喃唑酮、呋喃它酮、呋喃西林、呋喃妥因和硝夫索尔代谢物的同位素稀释液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry, LC-MS/MS)检测方法.方法 样品中加入同位素内标, 经盐酸水解及邻硝基苯甲醛衍生, 调节 pH为 7.2±0.2, 乙酸乙酯提取, 氮吹浓缩, 正己烷去脂净化, 色谱柱分离, 电喷雾电离(electronic spray ion, ESI)正负离子多反应(multiple reaction monitoring, MRM)模式检测, 同位素内标稀释法定量.结果 5种硝基呋喃代谢物的方法定量限为0.1~0.2 μg/kg; 方法的线性范围为0.2~20 μg/kg; 相关系数均大于0.999; 方法平均回收率为88.7%~112.1%, 相...

Objective To establish an analytical method for determination of furazolidone,furaltadone,nitrofurazone,nitrofurantoin and nifursol metabolites in shellfish by isotope dilution liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods Isotopic internal standards were added to the sample,which then were hydrolyzed by hydrochloric acid and derivatized with 2-nitrobenzaldehyde.The solution was neutralized to pH 7.2±0.2,extracted with ethyl acetate,and evaporated to dryness with nitrogen.The residue was reconstituted with mobile phase,and d...