期刊论文

中文

医学理论与实践

1001-7585

2017

30

17

2523-2525

10.19381/j.issn.1001-7585.2017.17.011

14B157:教育厅项目 16C1393:教育厅项目 16C1395:教育厅项目

本校为其他机构

目的：观察在APJ内源性配体13环境孕育下宫颈癌Caski细胞微核糖核酸-21的表达及细胞的增殖能力;分析Apelin-13和miR-21对宫颈癌Caski细胞的作用及意义。方法：通过qRT-PCR检测miR-21mRNA的表达情况和western blot检测PDCD4和Caspase-3蛋白的表达情况。细胞增殖能力采用CCK-8检测。结果：Apelin-13诱导miR-21的高表达并靶向调控下游基因PDCD4和Caspase-3的表达,从而促宫颈癌Caski细胞增殖。结论：Apelin-13通过调控miR-21高表达可能在宫颈癌Caski细胞中起关键性作用。

Objective：To detect the different expression of microRNA-21 amingcervical cancer cell line HeLa cells in exposure to distinct concentration of Apelin-13 andits effcet on cell proliferation.Methods：RT-PCR was used to detect the expression of microRNA-21 among cervical cancer cell line HeLa cells exposed to distinct concentration of Apelin-13.CCK-8was applied to check the capacity of cell proliferation.Expressions of Caspase-3and PDCD4 were detected by western blot.Results：Apelin-13 enhanced high expression of miR-21 and miR-21 regulated the t...