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A generic approach for expanding homolog-targeted residue screening of sulfonamides using a fast matrix separation and class-specific fragmentation-dependent acquisition with a hybrid quadrupole-linear ion trap mass spectrometer

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成果类型:
期刊论文
作者:
Huang, Chunlin;Guo, Bin;Wang, Xiaoying;Li, Jie;Zhu, Weitao;Chen, Bo;Ouyang, Shan;Yao, Shouzhuo
通讯作者:
Guo, B.
作者机构:
[Huang, Chunlin] Department of Biochemistry and Molecular Biology, School of Pharmacy and Life Science, University of South China, Hengyang 421001, China
通讯机构:
[Guo, Bin] Hunan Normal Univ, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.
Key Laboratory of Chemical Biology and Traditional, Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, China
语种:
英文
关键词:
Animal tissues;Dispersive solid-phase extraction;Multi-residue;Semi-targeted screening;Sulfonamides;Triple-quadrupole linear ion trap mass spectrometer
期刊:
Analytica Chimica Acta
ISSN:
0003-2670
年:
2012
卷:
737
页码:
83-98
基金类别:
National "863" Research Foundation [2010AA023001]; National Natural Science Foundation of China [20927005, 21005027]; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research [KLCBTCMR2009-06]; Hunan Provincial Education Department [10C1172]; Science Research Funds of Hunan province [2010TT1001]
机构署名:
本校为第一机构
院系归属:
化学化工学院
药学与生物科学学院
摘要:
A generic and efficient homolog-targeted approach was used to expand screening and detection of target class of sulfonamides and structural analogs, based on a fast single-tube extraction/partitioning-multifunction adsorption cleanup (SEP/MAC) for class-specific fragmentation-dependent acquisition with a liquid chromatography-hybrid triple-quadrupole linear ion trap mass spectrometer (LC-QqLIT). By combining the two-stage process conducted in a single tube as one-pot protocol, the straightforward SEP/MAC procedure was optimized to offer clean extracts with reasonable recovery (71-109% with RSDs < 20%) and decreased matrix interferences (-9 to 19%) of multiresidual sulfonamide extraction from different tissue samples. The novel use of neutral loss scan of 66 Da (NLS) or precursor ion scanning of m/z 108 (PreS) in positive ion mode was found to achieve more comprehensive coverage of protonated molecular ions of a wide array of sulfonamides including N-4-acetyl and hydroxylamine metabolites plus their possible dimers. Moreover, the PreS-triggered automatically enhanced product ion spectral acquisition enabled simultaneous screening, profiling and confirmation of an unlimited number of analytes belonging to the sulfonamide class within a single analysis. The validation and application results of the generic SEP/MAC-based LC-QqLIT strategy consistently demonstrated favorable performances with acceptable accuracy (67-116%), precision (RSDs < 25%), and sensitivity (LOQs <= 7.5 ng g(-1)) to meet the acceptance criteria for all the sulfonamide-tissue combinations. Thus, the integration of the matrix-independent SEP/MAC procedure and the multiparameter matching algorithm with the unit-resolution LC-QqLIT instrument can serve as a valuable semi-targeted discovery strategy for rapid screening and reliable quantitative/confirmatory analysis of real samples. (C) 2012 Elsevier B.V. All rights reserved.

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