期刊论文

中文

药物分析杂志

0254-1793

2022

42

2

287-293

10.16155/j.0254-1793.2022.02.13

湖南省自然基金青年基金（2018JJ3473）；

本校为第一机构

目的:探索石松的活性成分α -玉柏碱通过抗炎抗氧化作用对心肌缺血再灌注(MI/R)损伤的保护作用。方法: Wistar大鼠灌胃1 mL无菌水或含有2.5 mg·kg~(-1)或5 mg·kg~(-1) α -玉柏碱的水,持续1周,雷米普利1 mg·kg~(-1)作为阳性对照。结扎心脏左前降支30 min,再灌注3 h。超声检测射血分数EF(%) 、短轴缩短分数FS(%) 、左心室收缩压LVSP(mmHg) 、左心室舒张末期压力LVEDP(mmHg) 。伊文思蓝染指示心梗体积。试剂盒检测血清或心肌组织中总还原型谷胱甘肽(GSH) 、乳酸脱氢酶(LDH) 、天冬氨酸转氨酶(AST) 、超氧化物歧化酶(SOD)和丙二醛(MDA)的水平。ELISA测定血清肌酸激酶-同工酶(CK - Mb) 、肌钙蛋白...

Objective:To explore the protective effect ofα-obscurine,an active component of Lycopodium onmyocardial ischemia-reperfusion(MI/R)injury via anti-inflammatory and anti-oxidant ways.Methods:Wistar rats were intragastrically administered with 1 mL of sterile water or water containing 2.5 mg·kg-1 or 5 mg·kg-1α-obscurine for 1 week.Ramipril 1 mg·kg-1 was used as a positive control.The left anterior descending branch of the heart was ligated for 30 min and reperfused for 3 h.Echocardiograph was employed to test the the ejection fraction(EF,%),s...