期刊论文

中文

药物分析杂志

0254-1793

2022

42

3

380-386

10.16155/j.0254-1793.2022.03.03

湖南省自然科学基金项目（2018JJ6122）；

本校为其他机构

目的:探讨紫菀水提物对人肺癌A549细胞增殖、侵袭及裸鼠成瘤能力的影响及作用机制。方法:将体外培养MRC-5和A549细胞分别与不同浓度AWE(0、0.6、1.25、2.5、5、10、20、40、80、160、320 g·L~(-1))孵育,根据MTT实验设置A549细胞为AWE 0 g·L~(-1)组、AWE 10 ·L~(-1)组、AWE 20 ·L~(-1)组和AWE 40 · L~(-1)组。CCK-8法检测各组细胞增殖,Transwell法检测侵袭细胞数。采用Wnt/β-catenin信号通路抑制剂XAV939处理A549细胞,Western blot检测各组细胞Wnt-1、β-catenin、血管内皮生长因子(VEGF)和Ki-67蛋白表达。皮下种植A549细胞悬液(5×10~6个·mL~(-1)) 0.2 mL,经AWE(灌胃5 g·kg~(-1)·d~(-1...

Objective:To explore the effects and mechanisms of aster water extract(AWE)on the proliferationand invasion of human lung cancer A549 cells,and the tumorigenesis capability of nude mice.Methods:MRC-5 and A549 cells were cultured in vitro and incubated with different concentrations of AWE(0,0.6,1.25,2.5,5,10,20,40,80,160,320 g·L-1).In the MTT assay,A549 cells were divided into AWE 0 g·L-1 group,AWE 10 g·L-1 group,AWE 20 g·L-1 group and AWE 40 g·L-1 group,and the cell proliferation rate in each group was detected by CCK-8.The number of invas...