期刊论文

Screening of a Harposporium strain with high infectious protease activity and optimization of its fermentation conditions

中文

西北农林科技大学学报(自然科学版)

1671-9387

2019

47

7

118-124

10.13207/j.cnki.jnwafu.2019.07.016

31500014:国家自然科学基金 15C1218:湖南省教育厅科学研究项目 2016KS26:衡阳市科技计划

本校为第一机构

【目的】筛选钩丝孢属真菌高产蛋白酶菌株,并优化其产酶培养基和发酵条件,为寄生线虫病的生物防治提供优良菌种。【方法】以15株钩丝孢属真菌为材料,在28 ℃、180 r/min振荡培养10 d后,采用福林酚法测定其蛋白酶活性,筛选高产蛋白酶菌株,测定其产酶周期,并利用全齿复活线虫测定该菌株发酵液的杀线虫率。通过单因素试验和正交试验对高产蛋白酶菌株的产酶培养基及发酵条件进行优化,并对优化条件进行验证。【结果】从15株钩丝孢属真菌中筛选出1株高产蛋白酶菌株H6,其蛋白酶活性在发酵5 d最高。最佳产酶培养基成分为葡萄糖8 g/L、明胶8 g/L、蛋白胨8 g/L、酵母提取物4 g/L。最佳发酵条件为pH 7.0、2...

【Objective】 This study screened Harposporium strains with high protease activity and optimized the enzyme producing medium and fermentation conditions to provide basis for biological control of parasitic nematode.【Method】A total of 15 Harposporium strains were cultured for 10 days at 28 ℃ and 180 r/min oscillation. The protease activity was determined by Pollin phenol method. The strain with high protease activity was screened and the protease production cycle was confirmed. The nematocidal rate of its fermentation broth was tested by Pana...