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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Pu, Chunmin;Liao, Xiaoyan;Shi, Xianming;Cui, Yan;Bai, Yalong;...

通讯作者：

Chen, LL;Bai, YL

作者机构：

[Pu, Chunmin; Chen, Lili; Liao, Xiaoyan] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.

[Pu, Chunmin; Bai, Yalong; Liao, Xiaoyan] Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China.

[Shi, Xianming; Cui, Yan] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China.

通讯机构：

[Chen, LL ] U

[Bai, YL ] S

Univ South China, Coll Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.

Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China.

语种：

英文

关键词：

Agarose gel;Amino-modified magnetic particles;Aptamer;DNA extraction;Short single-stranded DNA

期刊：

International Journal of Biological Macromolecules

ISSN：

0141-8130

年：

2023

卷：

252

页码：

126500

DOI：

10.1016/j.ijbiomac.2023.126500

基金类别：

Acknowledgements This work was supported by Shanghai Agriculture Applied Technology Development Program (2022-02-08-00-12-F01089), Shanghai “Science and Technology Innovation Action Plan” for Agriculture Project (21N31901100), and Open Project of State Key Laboratory of Microbial Metabolism (MMLKF21-05).

机构署名：

本校为第一且通讯机构

院系归属：

公共卫生学院

摘要：

With the rapid advancements in aptamer screening, the efficient extraction of short single-stranded DNA (ssDNA) from agarose gel has become a new requirement. However, the currently available products are primarily designed for double-stranded DNA (dsDNA) and exhibit limited efficacy when applied to the extraction of short ssDNA. In this study, we successfully developed a novel method based on amino-modified silica-coated magnetic particles (ASMPs) for the extraction of short ssDNA from agarose gel. The gel slices containing short ssDNA were subjected to centrifugation in a spin column/centrif...

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