期刊论文

Yang, S.

中文

化学通报

0441-3776

2008

71

2

154-157

10.14159/j.cnki.0441-3776.2008.02.012

本校为第一且通讯机构

在25℃、pH 8.2的Tris-HCI缓冲液中,苏丹红I与人血清白蛋白(HSA)之间能产生有效的非辐射能量转移,苏丹红Ⅰ的加入使HSA的荧光猝灭.在此基础上,建立了通过人血清白蛋白共振能量转移荧光猝灭测定苏丹红I的方法,其方法线性范围为0.5～7.5μg/mL,检出限为0.1μg/mL,RSD=0.7%～1.8%,加标回收率为91.9%～109.3%.

It was found that the effective energy transfer could occur between Sudan I and Human Serum Albumin (HSA) in Tris-HCl buffer solution at pH 8.2, which quenched the fluorescence intensity of HSA. The fluorescence quenching intensity of HSA at 337 nm was proportional to the concentration of Sudan I. The linear range of the calibration curve was 0.5-7.5 μg/mL. The detection limit was 0.1 μg/mL, the ...