期刊论文

Luo, Z.-G.

中文

中国组织工程研究

2095-4344

2017

21

21

3394-3399

10.3969/j.issn.2095-4344.2017.21.019

国家自然科学基金面上项目（81273754）~~；

本校为第一且通讯机构

背景：利用未成熟树突状细胞诱导供体特异性免疫耐受有可能成为防治器官移植排斥反应的重要途径。目的：探讨青藤碱对大鼠来源骨髓树突状细胞体外分化及成熟的影响。方法：取大鼠股骨、胫骨骨髓来源树突状细胞的前体细胞,经粒-巨噬细胞集落刺激因子及白细胞介素4作用诱导出未成熟树突状细胞,第7天加入脂多糖刺激成熟,在刺激未成熟树突状细胞成熟时,分为对照组和低、中、高不同剂量的青藤碱处理组,作用48 h后收获树突状细胞,倒置相差显微镜观察细胞形态学特征,流式细胞仪检测细胞表型CD80、RT1B的表达,ELISA检测培养上清白细胞介素12水平,混合淋巴细胞反应检测树突状细胞刺激同种异体T淋巴细胞活...

BACKGROUND: It may be an important approach to avoiding organ transplant rejection by utilizing immature dendritic cells to induce donor-specific immunologictolerance. OBJECTIVE: To study the effect of sinomenine on the differentiation and maturation of rat bone marrow-derived dendritic cells in vitro. METHODS: IBone marrow-derived dendritic cells were isolated from the rat femur and tibia, and immature dendritic cells were induced by granulocyte-macrophage colony stimulating factor and interleukin-4. On day 7, lipopolysaccharide was added and ...