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持续静水压对细胞增殖的影响

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成果类型:
期刊论文
作者:
郭紫芬;冯勇;李凯;廖端芳
通讯作者:
Liao, D.-f.
作者机构:
[李凯; 郭紫芬; 冯勇; 廖端芳] Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
Guo Zi-FenSymbol, Doctor, Associate professor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum.
To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells.
Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively.
通讯机构:
[Liao, D.-f.] I
Institute of Pharmacy and Pharmacology, , Hengyang 421001, Hunan Province, China
语种:
中文
关键词:
高静水压;无血清细胞培养;血管内皮细胞;血管平滑肌细胞;增殖
期刊:
中国组织工程研究
ISSN:
2095-4344
年:
2011
卷:
15
期:
50
页码:
9353-9355
基金类别:
2008AA02Z436:国家高技术研究发展计划(863计划) 81102516:国家自然科学基金 CX2009B191:湖南省研究生创新项目
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
背景:血管平滑肌细胞在静水压替代血清培养的条件下能良好生长.目的:探讨无血清水平状态下,持续性静水压对细胞增殖的影响.方法:采用自行研制的压力可调细胞培养箱在0,12,16,20,24和28 kPa条件下培养血管平滑肌细胞A10与血管内皮细胞HUVEC-12.结果与结论:A10细胞在16 kPa压力的条件下增殖最明显,活性最强;HUVEC-12细胞在20 kPa压力的条件下增殖最明显,活性最强.说明一定范围内的静水压可促进体外培养的A10与HUVEC-12细胞增殖、使其活性增加.
摘要(英文):
BACKGROUND: Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum. OBJECTIVE: To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells. METHODS: Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively. RESULTS AND CONCLUSION: The most obvious proliferation of A10 cell was detected under the pressure of 16 kPa, with greatest ce...

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