[李凯; 郭紫芬; 冯勇; 廖端芳] Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
Guo Zi-FenSymbol, Doctor, Associate professor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum.
To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells.
Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively.
通讯机构:
[Liao, D.-f.] I
Institute of Pharmacy and Pharmacology, , Hengyang 421001, Hunan Province, China
BACKGROUND: Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum. OBJECTIVE: To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells. METHODS: Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively. RESULTS AND CONCLUSION: The most obvious proliferation of A10 cell was detected under the pressure of 16 kPa, with greatest ce...