期刊论文

中文

中国组织工程研究

2095-4344

2011

15

21

3843-3846

10.3969/j.issn.1673-8225.2011.21.010

湖南省衡阳市科委资金资助项目（2007KJ006）资助,项目名称:脱细胞同种异体神经的优化与组织工程神经的保存研究~~；

本校为第一机构

背景:脱细胞哺乳动物神经有有髓纤维直径小、细胞成分不易彻底清除、复合细胞不均匀的缺点。目的:制备脱细胞枪乌贼神经,并探讨其生物相容性。方法:用Hasse化学萃取法制备脱细胞枪乌贼神经,观察其脱细胞的效果、管道及基底膜的完整性,用MTT法检测脱细胞枪乌贼神经与细胞相容性,用皮下植入实验检测其组织相容性。结果与结论:制备的脱细胞枪乌贼神经细胞及髓鞘清除彻底、神经纤维膜管及基底膜保留完整,且膜管粗大。脱细胞枪乌贼神经皮下植入植入后1和2周材料周围有炎症细胞浸润。植入后4周材料周围有薄层纤维结缔组织囊,材料内部及周围只有少量炎症细胞。结果证实,采用Hasse化学萃取法制备的脱细胞...

BACKGROUND：Acellular nerves of mammals have disadvantages：①The diameter of myelinated fiber is small;②The cellular components are difficult to eliminate;③The cells are uneven when the acellular nerves are made into tissue engineering nerve.OBJECTIVE：To prepare a new kind of squid acellular nerve and to explore its biocompatibility.METHODS：The acellular nerves of squid were prepared by chemical methods（Haase chemical extraction）.The effects of cells removed were estimated.The integrity of the nerve fiber tube and Schwann cell basilar me...