期刊论文

Feng, L.

中文

中国组织工程研究

2095-4344

2010

14

20

3718-3721

10.3969/j.issn.1673-8225.2010.20.025

本校为第一且通讯机构

背景: 近年来动物实验和体外细胞培养研究证实促红细胞生成素对脑缺血具有神经保护作用, 有关促红细胞生成素脑保护的作用机制目前尚未阐明. 目的: 通过观察缺血损伤区域脑组织细胞学形态, 检测脑组织超氧化物歧化酶、丙二醛浓度, 探讨促红细胞生成素对脑缺血再灌注损伤的保护作用. 方法: 采用线栓法建立Wistar大鼠局灶性缺血再灌注损伤模型, 分别于缺血后2 h腹腔注射生理盐水3 000 U/kg、促红细胞生成素3 000, 1 000 U/kg, 并设假手术组. 缺血再灌注损伤24 h后, 应用苏木精-伊红染色法检测大鼠脑组织病理学变化, 应用黄嘌呤氧化酶法和硫代巴比妥酸法分别测定超氧化物歧化酶活性和丙二醛浓度. 结...

BACKGROUND: In recent years, animal experiments and in vitro cell culture studies confirmed that erythropoietin (EPO) has neuroprotective effect on cerebral ischemia, but the mechanism is not yet clearly. OBJECTIVE: To explore the neuroprotective effect of EPO on cerebral ischemia via observing cytologic morphology at damaged area and detecting concentrations of superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissues. METHODS: Wistar rats were prepared for focal ischemia-reperfusion models by suture method. At 2 hours after model preparation, 3 000 U/kg normal saline and 1000 U/k...