Dual-channel detection of metallothioneins and mercury based on a mercury-mediated aptamer beacon using thymidine-mercury-thymidine complex as a quencher
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21177052]; Science and Technology Program of Hunan Province in China [2010SK3039]; Construct Program of the Key Discipline (Public Health and Preventive Medicine) in Hunan Province
机构署名：
本校为第一且通讯机构
院系归属：
公共卫生学院
摘要：
A novel dual-channel strategy for the detection of metallothioneins (MTs) and Hg2+ has been developed based on a mercury-mediated aptamer beacon (MAB) using thymidine-mercury-thymidine complex as a quencher for the first time. In the presence of Hg2+, the T-rich oligonucleotide with a 6-carboxyfluorescein (TRO-FAM) can form an aptamer beacon via the formation of T-Hg2+-T base pairs, which results in a fluorescence quenching of the sensing system owing to the fluorescence resonance energy transfer (FRET) from the fluorophore of FAM to the terminated T-Hg2+-T base pair. The addition of MTs into ...
