期刊论文

Yang, F.

中文

中华麻醉学杂志

0254-1416

2022

42

3

328-332

10.3760/cma.j.cn131073.20210906.00317

湖南省教育厅***（19B477）； 湖南省卫健委课题（20200018, 20200037）； 湖南省自然科学基金（2021JJ70043）；

本校为其他机构

目的:评价脑源性神经营养因子反义长链非编码RNA(BDNF-AS)在大鼠神经病理性痛(NP)形成中的作用。方法:清洁级健康雄性SD大鼠，2月龄，体重200~260 g，采用左侧L 5-6脊神经结扎法制备NP模型。实验Ⅰ　取大鼠56只，采用随机数字表法分为3组：假手术组(Sham组， n＝8)、NP组( n＝24)和BDNF组( n＝24)。BDNF组于模型制备后1、3、6、13和20 d时双侧杏仁核注射外源性BDNF，每侧100 pmol。NP组和BDNF组于模型制备后7、14和21 d时随机取8只大鼠、Sham组于假手术模型制备后处死，取脑组织，分离杏仁核，ELISA法检测杏仁核BDNF含量，免疫组化法检测杏仁核BDNF阳性细胞数，实时定量PCR法检测杏仁核BDNF...

Objective:To evaluate the role of brain-derived neurotrophic factor-antisense long-chain non-coding RNA (BDNF-AS) in amygdala in the development of neuropathic pain (NP) in rats.Methods:Healthy clean-grade male Sprague-Dawley rats, aged 2 months, weighing 200-260 g, were used to develop NP model via ligation of left L 5-6 spinal nerve, while control group was only subjected to the exposure of L 5-6 spinal nerve without ligation.This study was performed in two parts.Experiment Ⅰ Fifty-six rats were divided into 3 groups by the random number...