期刊论文

Study on Preparation,Characterization and Cytotoxicity of Baicalin PEG-PE Nanomicelles

中文

中国药房

1001-0408

2019

30

11

1487-1491

10.6039/j.issn.1001-0408.2019.11.10

湖南省卫生计生委科研计划课题（No.20180059）；

本校为第一机构

目的:制备黄芩苷(BAI)聚乙二醇衍生化磷脂酰乙醇胺(BAI@PEG-PE)纳米胶束,并对其进行表征和细胞毒性研究。方法:采用薄膜水化法制备BAI@PEG-PE 纳米胶束,观察其外观特征,检测其粒径、多分散系数(PDI)、Zeta 电位、载药量、包封率。比较BAI 原料药和BAI@PEG-PE 纳米胶束在pH 7.4 磷酸盐缓冲液中1~84 h 内的释药情况。以香豆素6 为荧光探针,观察PEG-PE纳米胶束在H9c2 心肌细胞中的分布。将H9c2 心肌细胞分为模型组、BAI原料药组和BAI@PEG-PE纳米胶束组,用不含药或含相应药物的无血清DMEM 培养液培养0.5 h 后,用异丙肾上腺素诱导心肌细胞凋亡,比较3 组细胞的细胞核形态变化、细胞凋亡率和凋亡相关蛋...

OBJECTIVE:To prepare Baicalin-loaded Polyethylene glycol-derivatized phosphatidylethanolamine(BAI@PEG-PE) nanomicelles,and to characterize it and study its cytotoxicity. METHODS:BAI@PEG-PE nanomicelles were prepared by film hydration method and their appearance characteristics were observed. The particle size,polydispersity index,Zeta potential, drug-loading amount and encapsulation efficiency of the nanomicelles were detected. Drug release of BAI raw material and BAI@PEG-PE nanomicelles in pH 7.4 phosphate buffer were compared within 1-84 h. U...