An Assay for the c. 4012 C > T of Adenomatous Polyposis Coli Hotspot Mutation Using a Strategy Combining Restriction Digestion and Blue/White Screening Technology
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81301267]; Jiangsu Province's clinical medical science and technology program [BL2013016]
机构署名：
本校为其他机构
院系归属：
医学院
摘要：
APC gene mutations are associated with the development of many types of cancers and also related to cancers' sensitivities to selected drugs. Genetic testing of APC mutation using cancer tissues have been well developed, but assays targeting circulating tumor DNA of APC gene mutations are to be developed in order to meet the requirements by clinical application. This study tested the possibilities of applying restriction enzyme digestion combined with blue/white screening technology in analyzing a hotpot point mutation in APC gene. The present study has documented that the combination of restr...
