期刊论文

The Fos protein levies in central nervous system in rats exposed to methyl tertiary butyl ether

中文

右江民族医学院学报

1001-5817

2008

30

1

3-6

10.3969/j.issn.1001-5817.2008.01.002

湖南省卫生厅科研项目基金[B2005090]；

本校为第一机构

目的 通过研究甲基叔丁基醚(MTBE)对大鼠中枢神经系统Fos蛋白的影响,探讨MTBE对中枢神经系统急性毒作用部位.方法 54只健康成年SD大鼠,体重180～220 g,随机分为9组,腹腔注射MTBE原液染毒,以未染毒为对照,染毒剂量分别为700、1050、1400mg(kg·bw),染毒后用免疫组织化学方法检测Fos蛋白的表达并用相对灰度值定量.结果 未染毒对照组脑组织神经元内仅有少量Fos蛋白表达,MTBE急性染毒后在大脑皮质、丘脑、下丘脑、小脑皮质神经元内Fos蛋白表达均有明显增多,Fos蛋白表达的相对灰度值随着MTBE染毒剂量的增加,其表达逐渐增强,其中MTBE对大脑皮质、小脑皮质的毒作用较丘脑、下丘脑更为明显;各部位Fos蛋白...

Objeetive To investigate the effects of methyl tertiary butyl ether （MTBE） on the FOS protein in central nervous system of the rats. Methods 54 SD healthy adultrats weighed 180-220g were involved in this study. They were randomly divided into 9 groups and were given intrabdominal MTBE injection. The MTBE exposure dosages were 700, 1050, and 1400mg/（kg· bw）. After the rats were exposed to MTBE, an immunohistochemical method was used to detect the FOS protein levels and the Grayvalue was determined. The control group was given normal saline....