期刊论文

Guo, Y.

中文

中国药理学通报

1001-1978

2003

19

4

401-403

10.3321/j.issn:1001-1978.2003.04.010

国家自然科学基金课题No 39970 84 7 湖南省卫生厅中医药科研课题No 2 0 0 0 0 0 6

本校为第一且通讯机构

目的：探讨金粉蕨素拮抗Menadione氧化损伤所抑制的内皮细胞增殖的作用及其机制。方法：以Menadione(O2^-)损伤人脐静脉内皮细胞作为氧化损伤模型，采用MTT法和细胞计数法，观察不同浓度金粉蕨素对Menadione损伤内皮细胞生长抑制率的影响；利用硝酸还原酶法测定培养液中NO含量；以Western blot检测细胞eNOS活性及磷酸化ERK1／2的表达。结果：金粉蕨素保护组与损伤组相比，内皮细胞的生长抑制率明显降低，培养液中NO含量增高，eNOS活性增强，磷酸化ERK1／2表达上调。结论：NO和ERK1／2通路可能介导了金粉蕨素拮抗Menadione氧化损伤所抑制内皮细胞增殖的保护作用。

AIM: To investigate the protective action of onychin aganist the growth inhibition of endothelial cell injured by menadione and its mechanism. METHODS: The injured model was established by endothelial cell treated with menadione. Protective effect of onychin aganist growth inhibition of injured endothelial cell was determined by MTT assay and cell counting method; NO concentration in the medium was determined by nitrate reductase assay; eNOS and phosph-ERK1/2 protein levels were determined by Western blot. RESULT: Onychin significantly decreased the growth inhibitory rate of injured endothelia...