期刊论文

中文

中国比较医学杂志

1671-7856

2007

17

6

341-344

10.3969/j.issn.1671-7856.2007.06.008

本校为第一机构

目的 探讨丙泊酚的遗传毒性及对遗传物质可能产生的损伤作用.方法 应用鼠伤寒沙门氏菌回复突变实验、单细胞凝胶电泳实验及小鼠骨髓微核实验,测丙泊酚对基因突变、染色体改变和原发性DNA损伤3个遗传学终点的影响.结果 丙泊酚在每皿1.0 mg～0.01 mg范围内, 有或无代谢活化系统时, TA97、TA98、TA100和TA102 4种菌株的每皿平均回变菌落数未见阳性的剂量-反应关系.单细胞凝胶电泳实验:小鼠经丙泊酚1.5 mg/kg、3.0 mg/kg 、6.0 mg/kg 和12.0 mg/kg染毒后外周血淋巴细胞彗星率分别为4%,6%,5%和6%;彗星尾长分别为:(21.51±0.48) μm、(21.67±0.58) μm、(21.89±0.48) μm、(22.01±0.66) μm.各组彗...

Objective To assess the genotoxicity activity of propofol. Methods Three genotoxic tests, i.e. Ames, single cell gel electrophoresis （SCGE）, and micronucleus assays were used in this study. Ames test was applied to detect gene mutation whenSalmoneUa typhimuriumwere exposed to propofol. Single cell gel electrophoresis （SCGE） assay was used to detect DNA single strands breaks （DNA-SSBs） when rats were exposed to propofol at different concentrations. The rate of micronuleus influenced by propofol was measured in rat bone marrow cells. Result...