Although an affinity tag such as six consecutive histidines, (His)6-tag, has been widely used to obtain high quantity of recombinant proteins, little is known about its influences on heme proteins for lack of structural information. When (His)6-tag was introduced to the N-terminus of a small heme protein, cytochrome b
5, experimental results showed the resultant protein, (His)6-cyt b
5, has similar property and function to that of isolated cyt b
5. To provide structural information for this observation, we herein performed a structural prediction of (His)6-cyt b
5 by molecular modeling in ...