期刊论文

中文

中国病理生理杂志

1000-4718

2024

40

5

852-861

10.3969/j.issn.1000-4718.2024.05.010

No.91839103:国家自然科学基金 No.81670429:国家自然科学基金 No.2020SK2105:湖南省重点研发计划

本校为第一且通讯机构

目的:探究mitoNEET能否抑制铁死亡引起的棕色脂肪细胞线粒体代谢功能紊乱.方法:采用原代C57BL/6J小鼠棕色脂肪细胞构建体外模型,Western blot检测相关蛋白,试剂盒检测细胞中铁离子和ATP含量,荧光显微镜及流式细胞术检测线粒体膜电位和活性氧(ROS)水平.结果:与对照组相比,铁死亡诱导剂erastin处理组棕色脂肪细胞中铁死亡相关蛋白ACSL4表达增加了1.13倍,SLC7A11和GPX4表达分别减少了27.33%和25.33%(P

AIM:To investigate the potential impact of mitoNEET[mitochondrial protein containing Asn-Glu-Glu-Thr(NEET)sequence]on mitochondrial metabolism in brown adipocytes,and to elucidate its underlying mecha-nism. METHODS:An in vitro model of primary mouse brown adipocytes was established. Western blot were utilized to detect relevant proteins,and iron ion and ATP content was measured using kits. Mitochondrial membrane potential and re-active oxygen species(ROS)were assessed by fluorescence microscopy and flow cytometry. RESULTS:The expression of the ...