期刊论文

Overexpression of mitoNEET inhibits ferroptosis-induced mitochondrial metabolic dysfunction in brown adipocytes

中文

中国病理生理杂志

1000-4718

2024

40

5

852-861

10.3969/j.issn.1000-4718.2024.05.010

No.91839103:国家自然科学基金 No.81670429:国家自然科学基金 No.2020SK2105:湖南省重点研发计划

本校为第一机构

目的:探究mitoNEET能否抑制铁死亡引起的棕色脂肪细胞线粒体代谢功能紊乱.方法:采用原代C57BL/6J小鼠棕色脂肪细胞构建体外模型,Western blot检测相关蛋白,试剂盒检测细胞中铁离子和ATP含量,荧光显微镜及流式细胞术检测线粒体膜电位和活性氧(ROS)水平.结果:与对照组相比,铁死亡诱导剂erastin处理组棕色脂肪细胞中铁死亡相关蛋白ACSL4表达增加了1.13倍,SLC7A11和GPX4表达分别减少了27.33%和25.33%(P

AIM:To investigate the potential impact of mitoNEET[mitochondrial protein containing Asn-Glu-Glu-Thr(NEET)sequence]on mitochondrial metabolism in brown adipocytes,and to elucidate its underlying mecha-nism.METHODS:An in vitro model of primary mouse brown adipocytes was established.Western blot were utilized to detect relevant proteins,and iron ion and ATP content was measured using kits.Mitochondrial membrane potential and re-active oxygen species(ROS)were assessed by fluorescence microscopy and flow cytometry.RESULTS:The expression of the ferr...