期刊论文

中文

中国病理生理杂志

1000-4718

2022

38

5

769-778

10.3969/j.issn.1000-4718.2022.05.001

国家自然科学基金资助项目（No.81973326）；

本校为第一机构

目的:研究Rab18是否通过Janus激酶2(JAK2)/信号转导及转录激活因子3(STAT3)信号通路下调脂滴包被蛋白2(PLIN2)表达,并影响人THP-1巨噬细胞脂质蓄积。方法:采用氧化低密度脂蛋白(ox-LDL)孵育人THP-1巨噬细胞不同时间(0、6、12和24 h),用Western blot检测细胞内Rab18、JAK2、STAT3、p-JAK2、p-STAT3及PLIN2蛋白水平,使用油红O染色法观察细胞内脂质蓄积的变化。制备野生型、活化型和失活型Rab18巨噬细胞,予以ox-LDL孵育,Western blot和RT-qPCR检测细胞内Rab18、JAK2、STAT3、p-JAK2、p-STAT3及PLIN2蛋白水平,细胞免疫荧光技术观察巨噬细胞内p-JAK2和p-STAT3的核转位情况。用JAK2抑制剂AG490预处理活...

AIM:To investigate the effect of Rab18 on the accumulation of lipids in human THP-1 macrophages.METHODS:Human THP-1 macrophages were co-incubated with oxidized low-density lipoprotein(ox-LDL)for different time(0,6,12 and 24 h).Western blot was used to detect the protein levels of Rab18,Janus kinase 2(JAK2),signal transducer and activator of transcription 3(STAT3),p-JAK2,p-STAT3 and perilipin 2(PLIN2)in the cells.Oil red O staining was used to observe the intracellular lipid changes.Wild-type,high-activity and low-activity Rab18 cells were prepared and incubated with ox-LDL.Western blot and RT-...