期刊论文

PLIN2 promotes lipid accumulation in RAW264.7 macrophages by regulating SREBP2

中文

中国病理生理杂志

1000-4718

2021

37

01

1-9

10.3969/j.issn.1000-4718.2021.01.001

国家自然科学基金资助项目（No.81973326）；

本校为第一机构

目的:探讨脂滴包被蛋白2(PLIN2)是否通过调控固醇调节元件结合蛋白2(SREBP2)影响小鼠RAW264.7巨噬细胞中脂质蓄积。方法:采用氧化低密度脂蛋白(ox-LDL)处理RAW264.7巨噬细胞不同时间(0、6、12、24和48 h),Western blot检测PLIN2和SREBP2蛋白表达水平;过表达或敲减PLIN2,RT-qPCR和Western blot检测SREBP2、3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR)和葡萄糖调节蛋白78(GRP78)表达水平,油红O染色检测细胞脂质蓄积情况;采用免疫荧光和氧化酶法分别检测PLIN2对SREBP2核转位和细胞内游离胆固醇(FC)的影响;采用内质网应激(ERS)抑制剂4-苯基丁酸(4-PBA)处理细胞,Western blot和RT-qPCR检测GRP78、SREBP2和...

AIM:To investigate whether perilipin 2(PLIN2)promotes lipid accumulation by regulating the expression of sterol regulatory element binding protein 2(SREBP2)in mouse RAW264.7 macrophages.METHODS:Oxidized low-density lipoprotein(ox-LDL)was used to treat RAW264.7 macrophages for different time(0,6,12,24 and 48 h),and Western blot was used to detect the protein levels of PLIN2 and SREBP2.The expression levels of SREBP2,3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGCR)and glucose-regulated protein 78(GRP78)were detected by RT-qPCR and Western blot in RAW264.7 macrophages withPLIN2 over-express...