期刊论文

中文

中国病理生理杂志

1000-4718

2018

34

7

1189-1194

10.3969/j.issn.1000-4718.2018.07.006

湖南省教育厅优秀青年科研项目（No.14B183）；

本校为其他机构

目的:探讨adipophilin促进细胞内脂质蓄积的可能机制,为动脉粥样硬化的防治提供参考。方法:分别通过qPCR、Western blot和油红O染色观察氧化型低密度脂蛋白(oxLDL)处理RAW264.7细胞不同时间后,细胞内Akt、p-Akt和adipophilin的蛋白水平及脂质蓄积情况;并检测PI3K/Akt抑制剂LY294002处理后,上述指标的变化;HEK293细胞过表达adipophilin后,检测Akt的活性;并用免疫共沉淀实验检测adipophilin与Akt之间的相互作用。结果:oxLDL处理细胞后,随着时间的延长,脂滴增多,Akt被活化,adipophilin表达增加,而LY294002处理则可抑制上述变化;过表达adipophilin后,p-Akt水平增高,但adipophilin与Akt之间未见直接相...

AIM： To observe the possible mechanism through which adipophilin promotes the accumulation of intracellular lipids,and to provide a reference for controlling atherosclerosis. METHODS： RAW264. 7 cells were incubated with oxidized low-density lipoprotein（ ox LDL） for different time. q PCR,Western blot and Oil red O staining were used to observe the mRNA and protein levels of Akt,p-Akt and adipophilin and lipid accumulation. The above indexes were measured after the cells were treated with PI3 K/Akt signaling pathway inhibitor LY294002. The ac...