期刊论文

中文

中国病理生理杂志

1000-4718

2005

21

12

2362-2365

10.3321/j.issn:1000-4718.2005.12.017

纽约中华医学基金资助项目（NO．CMB 82-412）；

本校为其他机构

目的:探讨肥胖者血清载脂蛋白E基因多态性与HDL亚类组成的关系.方法:采用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析93例肥胖者和96例非肥胖者者的apoE基因型、HDL各亚类组成及相对含量.结果:肥胖组和对照组apoE基因型及等位基因频率分布均以E3/3和ε3最高.肥胖者等位基因ε2携带者血清apoE/CⅢ、HDL2a较等位基因ε3和ε4携带者升高,而apoB100、apoCⅢ、HDL3.则较ε3携带者下降,差异显著(P＜0.05).对照组中等位基因ε2携带者血清TC、apoE较等位基因ε3携带者升高,等位基因ε2携带者HDL3b较等位基因ε3携带者降低,差异显著(P＜0.05).结论:apoE基因多态性与HDL亚类的组成和...

AIM： The purpose of this study is to elucidate the relationship between apolipoprotein （apo） E polymorphism and plasma lipid profiles and HDL subclasses in obesity. METHODS： apoE genotype was assayed by polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP）. The subclasses of senun HDL in 93 obese subjects and 96 nonobese subjects were determined by two- dimensional gel electrophoresis conjunction with immunodetection method. RESULTS： apoE3/3 genotypes and allele ε3 frequency in obese group and the control gr...